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Saturday, November 6, 2021

QUESTIONS ON BIOTECHNOLOGY

 CHAPTER 12:- BIOTECHNOLOGY

1) Define Genetic Engineering.

2) Who is known as father of genetic engineering?

3) Define Biotechnology.

4) What are sticky ends?

5) What are palindromes? Give one example.

6) What are the two types of gene library?

7) Which is the most widely used vector for producing Bt crop.

8) Name the different enzymes involved in genetic engineering.

9) What is the actual role of restriction enzymes?

10)    What is ligation?

11)    What are bacteriophages? Give their examples.

12)    Name the widely used phage vector.

13)    What are transposons?

14)    Who discovered polymerase chain reaction?

15)    What is restriction site?

16)    Define transfection.

17)    Why screening of cDNA clone is not necessary?

18)    What is cryopreservation?

19)    What is bio-piracy?

20)   Which material is used for coating DNA particles in gene gun method?

21)    Which enzymes reduces shelf life of tomato?

22)   Give one use of PCR technique.

23)   Name the bacterium from which enzyme is isolated for PCR technique.

24)   Explain Hind III.

25)   What do you mean by pBR 322?

26)   Define transfection?

27)   What is cosmid?

28)   Define plasmids.

29)   Who proposed recombinant DNA technique.

30)   Mention any two applications of biotechnology.


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